Fig.5.4 Micrococcal nuclease analysis of chromatin structure.

MNase digestion of liver nuclei was followed by DNA purification and separation on an agarose gel by electrophoresis. A Southern blot of the gel was probed for the presence of the alpha-fetoprotein gene (S. Stratton and M.C. Barton, unpublished results). Increasing amounts of MNase (denoted by the triangle) used in each digestion reaction leads to progressive degradation of DNA in the linker regions, unprotected by nucleosomes (lanes 1-4). The diagram to the left illustrates the amount of DNA (solid line) at each position of the blot, protected by nucleosome(s) (dashed oval) from MNase cleavage.

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