Concluding Remarks

Although Rb has been extensively studied since its identification as the first tumor suppressor gene, much remains to be discovered about both Rb and E2F's physiological roles and the specific mechanisms of action that account for those roles. Recent molecular discoveries have begun to provide a mechanistic explanation for the observation that some target genes are controlled by specific E2F family members, and further understanding of the specific interactions between E2F/Rb family members and other transcription factors may also explain complex patterns of timing and levels of induction.- The mechanisms and targets found in biochemical and microarray experiments will need to be examined in vivo to determine which interactions and targets are crucial for determining the physiological outcome. This information can be combined with advances in mouse targeting constructs and micro-array techniques which have already expanded the arenas in which Rb and E2F are known to play key roles.

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