The substrate of the enzyme trehalase is trehalose, commonly called mushroom or fungal sugar. It is a disaccharide composed of two glucose units joined by an a,1^1 glycosidic bond (Figure 10.8). Remarkable effects of trehalose on the protection of membranes and thermostabilization of enzymes has been found (Crowe et al., 1992). Bharadwaj and Maheshwari (1999) chose the enzyme trehalase (which hydrolyzes trehalose) for a comparative investigation in a thermophilic and a mesophilic fungus. Thermomyces lanuginosus grows optimally between 50 and 55°C, whereas Neurospora crassa grows optimally

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