Bartnicki-Garcia, S. (2002). Hyphal tip growth: outstanding questions. In: Osiewacz, H.D., ed.,

Molecular Biology of Fungal Development. New York: Marcel Dekker, pp. 29-58. Brasier, C.M. (1984). Inter-mycelial recognition systems in Ceratocystis ulmi, pp. 451-497. In: Jennings, D.H. and Rayner, A.D.M., eds., The Ecology and Physiology of the Fungal Mycelium. Cambridge: Cambridge University Press.

Burnett, J.H. (1976). Fundamentals of Mycology. London: Edward Arnold, Chapter 3.

Carlile, M.J. (1995). The success of hypha and mycelium. In: Gow, N.A.R. and Gadd, G.M., eds., The Growing Fungus. London: Chapman and Hall, pp. 3-19.

Dynesen, J. and Nielsen, J. (2003). Branching is coordinated with mitosis in growing hyphae of Aspergillus nidulans. Fungal Genet. Biol. 40:15-24.

Gooday, G.W. and Gow, N.A.R. (1990). Enzymology of tip growth in fungi. In: Heath, I.B., ed., Tip Growth in Plant and Fungal Cells. New York: Academic Press, pp. 31-58.

Gupta, G.D. and Heath, I.B. (2000). Tip-high gradient of a putative plasma membrane SNARE approximates the exocytotic gradient in hyphal apices of the fungus Neurospora crassa. Fungal Genet. Biol. 29:187-199.

Gupta, G.D. and Heath, I.B. (2002). Predicting the distribution, conservation, and functions of SNAREs and related proteins in fungi. Fungal Genet. Biol. 36:1-21.

Harold, F.M. (1999). In pursuit of the whole hypha. Fungal Genet. Biol. 27:128-133.

Heath, I.B., Gupta, G. and Bai, S. (2000), Plasma membrane-adjacent actin filaments, but not microtubules, are essential for both polarization and hyphal tip morphogenesis in Saprolegnia ferax and Neurospora crassa. Fungal Genet. Biol. 30:45-62.

Hickey, P.C., Jacobson, D.J., Read, N.J. and Glass, L. (2002). Live-cell imaging of vegetative hyphal fusion in Neurospora crassa. Fungal Genet. Biol. 37:109-119.

Hyde, G.J. and Heath, I.B. (1997). Ca2+ gradients in hyphae and branches of Saprolegnia ferax. Fungal Genet Biol. 21:238-251.

Jaffe, L.F. and Nuccitelli, R. (1974). An ultrasensitive vibrating probe for measuring steady electrical currents. J. Cell Biol. 63:614-628.

Kropf, D.L., Caldwell, J.H., Gow, N.A.R. and Harold, F.M. (1984). Transcellular ion currents in the water mold Achlya. Amino acid proton symport as a mechanism of current entry. J. Cell Biol. 99:486-496.

Levina, N.N., Lew, R.R., Hyde, G.J. and Heath, I.B. (1995). The roles of Ca2+ and plasma membrane ion channels in hyphal tip growth of Neurospora crassa. J. Cell Sci. 108:3405-3417.

Lopez-Franco, R., Bartnicki-Garcia, S. and Bracker, C.E. (1994). Pulsed growth of fungal hyphal tips. Proc. Natl. Acad. Sci. USA 91:12228-12232.

Riquelme, M., Reynaga-Pena, C.G., Gierz, G. and Bartnicki-Garcia, S. (1998). What determines growth direction in fungal hyphae? Fungal Genet. Biol. 24:101-109.

Rothman, J.E. (1994). Mechanisms of intracellular protein transport. Nature 372:55-63.

Silverman-Gavrila, L.B. and Lew, R.R. (2000). Calcium and tip growth in Neurospora crassa. Protoplasma 213:203-217.

Silverman-Gavrila, L.B. and Lew, R.R. (2001). Regulation of the tip-high [Ca2+] gradient in growing hyphae of the fungus Neurospora crassa. Eur. J. Cell Biol. 80:379-390.

Smith, M., Bruhn, J.N. and Anderson, J.B. (1992). The fungus Armillaria bulbosa is among the largest and oldest living organisms. Nature 356:428-431.

Talbot, N.J. (1997). Growing into the air. Curr. Biol. 7:78-81.

Ward, M. (2002). Expression of antibodies in Aspergillus niger. Genetic Engineering News 22 (

Wessels, J.G.H. (1996). Fungal hydrophobins: proteins that function at an interface. Trends Plant Sci. 1:9-15.

Wolkow, T.D., Harris, S.D. and Hamer, J.E. (1996). Cytokinesis in Aspergillus nidulans is controlled by cell size, nuclear positioning and mitosis. J. Cell Sci. 109:2179-2188.

Wösten, H.A.B., Moukha, S.M., Sietsma, J.H. and Wessels, J.G.H. (1991). Localization of growth and secretion of proteins in Aspergillus niger. J. Gen. Microbiol. 137:2017-2023.

Wösten, H.A.B., Schuren, F.H.J. and Wessels, J.G.H. (1994). Interfacial self-assembly of a hydro-phobin into and amphipathic membrane mediates fungal attachment to hydrophobic surfaces. EMBO J. 13:5848-5854.

Chapter 2

The Multinuclear Condition

Fungal nuclei measure about 2 to 3 ^m in diameter and in the past their small size and poor staining by the conventional cytological stains discouraged their studies. However, recently several DNA-specific fluorescent dyes have been used for routine observations (Figure 2.1). In the last few years, a Neurospora chromosomal protein gene, histone H1, was tagged with a gene from a jellyfish that encodes a green fluorescent protein and glows bright green under blue light excitation (488 nm), thus making visualization of fungal nuclei possible under a fluorescence microscope. This method allows nuclei to be viewed in unfixed hyphae and to study the long distance movement of nuclei in living hyphae by video microscopy. The ease of isolating mutants has stimulated studies on the identification of nuclear genes involved in hyphal growth and morphogenesis. The feasibility of fusing fungal cells containing two different nuclear types into heter-okaryons makes possible the study of the cooperation or competition among nuclei in multinuclear hypha. Electrophoresis allows the separation of the tiny fungal chromosomes and makes the determination of the chromosome numbers more reliable than was possible by squash preparations.

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