O

+ Hygromycin

+ Hygromycin ;

Conidial plating

+ Hygromycin ;

Conidial plating

% Hygr colonies = 30

Figure 9.7 Diagram of silencing of hph transgene in Neurospora crassa. Based on Pandit and Russo (1992).

gene silencing. These terms all mean the same thing, i.e., the mutual inactivation of gene expression by homologous sequences. The phenomenon has received more attention and publicity in plants because the expression of transgenes is necessary for crop improvement. However, Neurospora has provided the best clues because of the opportunity of investigating the involvement of a trans-acting diffusible molecule using heterokaryons. The

Isopentenyl-PP

0 0

Post a comment