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Figure 14.8 Diagram of restriction fragment patterns from mitochondrial DNA of wild (nd+), nd mutant and a [nd+ + nd] heterokaryon. Adapted from Bertrand et al. (1993).

phenotype upon subculturing (Figure 14.12). In crosses of senescent X wild type, the mutant and wild type segregated in a 1:1 ratio (Figure 14.13), demonstrating that senescent is a single nuclear-gene mutant. Comparison of restriction fragments of mitochondrial DNAs from sen+ showed that senescing mycelia suffered deletions and gross sequence rearrangements.

Figure 14.9 Diagram of unequal crossing over between repeat sequences. Based on Bertrand et al. (1993).

Multinucleate cell

Multinucleate cell

Induced microconidiation

Uninucleate microconidia

Induced microconidiation

Homokaryotic colonies

Plating of microconidia and isolation of individual colonies

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