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-2.3 kb

Figure 9.4 Discovery of RIP phenomenon. Detection and modification of transforming sequences in the am+ progeny from the cross of transformant X host DNA was digested with BamHI, fractionated and probed with the am region. The appearance of a novel 10 kb band in the am- progeny indicated modification or deletion of BamHI sites b, c and d in the duplicated regions of the progeny. The am+ progeny retained all four regions of the plasmid. Based on Selker et al. (1987).

Figure 9.4 Discovery of RIP phenomenon. Detection and modification of transforming sequences in the am+ progeny from the cross of transformant X host DNA was digested with BamHI, fractionated and probed with the am region. The appearance of a novel 10 kb band in the am- progeny indicated modification or deletion of BamHI sites b, c and d in the duplicated regions of the progeny. The am+ progeny retained all four regions of the plasmid. Based on Selker et al. (1987).

genes immune to RIP are the tandemly repeat units of rDNA, presumably protected because of their localization in nucleolus.

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