Zambonis Solution

1. Indications. General purpose fixative. Allows secondary fixation with osmium, which makes it suitable as a primary fixative for electron microscopy.

2. Composition. Stock solution: 20.0 g paraformaldehyde, 150 mL saturated aqueous (double filtered) picric acid.

Heat to 60°C. After the paraformaldehyde is dissolved, add drops of 2.5% aqueous sodium hydroxide to render the solution alkaline. Filter solution and allow to cool.

Add phosphate buffer to solution to make 1,000 mL. Composition of phosphate buffer: 3.32 g monobasic sodium phosphate (NaH2PO4H2O), 17.88 g dibasic anhydrous sodium phosphate (Na2HPO4), 1,000 mL distilled water.

If the final pH is not 7.3, the value must be adjusted.

3. Procedure and storage. Similar to formalin.

Reducing Blood Pressure Naturally

Reducing Blood Pressure Naturally

Do You Suffer From High Blood Pressure? Do You Feel Like This Silent Killer Might Be Stalking You? Have you been diagnosed or pre-hypertension and hypertension? Then JOIN THE CROWD Nearly 1 in 3 adults in the United States suffer from High Blood Pressure and only 1 in 3 adults are actually aware that they have it.

Get My Free Ebook

Post a comment