Preparation Of Undecalcified Sections And Microradiography

Bone specimens fixed in buffered formalin are dehydrated in alcohol, as in routine histologic preparations, and then embedded in methyl methacrylate (8). Specimens are cut to a thickness of 75-125 pm, usually with a diamond or carborundum-embedded wheel. For quantitative microradiography, sections are ground to a thickness of 100 pm (±5 pm). Kodak highresolution plates (Eastman Kodak Company, Rochester, NY) can be used, preferably with a vacuum cassette, which ensures that the specimen is placed flat against the emulsion (9). Unde-calcified bone can also be prepared for electron microscopy (10).

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