Pheochromocytoma

1. Procedure. Place a few drops of Zenker's fixative stock solution (see page 131), without glacial or formic acid, on a slice of fresh tumor tissue. A pheochromocytoma containing adrenaline or noradrenaline or both will turn brown in less than 20 min. Light of a photolamp will accelerate the reaction. Tissue is subsequently fixed in 10% buffered neutral formalin solution. For histologic demonstration of chromaffin granules in these tumors, the tissue should be fixed in Orth's solution. (See above under "Fixation for Light Microscopy."

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Your Heart and Nutrition

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