Papermounted Sections

This method was pioneered by Gough and has undergone several modifications (10). The technique yields very instructive, detailed, esthetically appealing, and extremely durable views of pulmonary abnormalities. After perfusion fixation with formalin and sodium acetate, 2-cm thick slices of the lungs are washed and embedded in a gelatin mixture that contains a disinfectant. After the gelatin mixture has penetrated the tissue, the block is frozen and large, 400-pm sections are cut, refixed, and transferred to another gelatin mixture, and eventually mounted on paper. Routine stains can be applied without difficulty. The technique also can be applied to other organs such as liver. For further details, the second edition of this book

Fig. 4-6. Slice of perfusion-fixed lung. The left lung was cut in a sagittal plane. The slice shown was impregnated under water with barium sulfate as described by Heard (see text). Note multiple tubular bronchiectases.

(8) or the original publications should be consulted. Readers will find that paper-mounting requires some skill; it also is work-intensive (the original method of Gough required 11 d, although other authors have achieved comparable results in

Fig. 4-7. Arteriogram of left lung. Lung inflated with carbon dioxide and pulmonary artery injected with barium sulfate-gelatin mixture. Note marked rarefication of vascular tree of this emphysematous lung.

2 d [11]), and thus costly. Despite its didactic and esthetic appeal, the method has been largely replaced by photography of perfusion-fixed specimens.

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