Pancreas

The parenchyma usually is best exposed by cutting the organ in the frontal plane. Parallel sagittal sections are preferred when the pancreatic duct is dilated. When only one routine section of pancreatic tissue is to be studied, the tail of the pancreas is usually selected because of the abundance of islets in this region. For the demonstration of lipomatosis, a slice can be stained with Sudan III (see page 133). For the accentuation of fat tissue necroses, a slice of formalin-fixed tissue is placed in a copper acetate solution. After 1 d in the incubator or several days at room temperature, the fat tissue necroses turn blue green (12).

Arteriograms require injection of the celiac and superior mesenteric artery system, as described earlier. The retrograde

Fig. 5-6. Preparation for angiography and cholangiography. (A) Straight and bifurcated nozzles for hilar vessels and bile ducts; rubber hose for attaching specimens to perfusion apparatus, cotton wads for plugging hepatic veins, and ligature with needle to secure nozzles. Two identification tags are also shown. (B) Cirrhotic liver with nozzle tied into portal vein. Adapted with permission from ref. (10).

Fig. 5-6. Preparation for angiography and cholangiography. (A) Straight and bifurcated nozzles for hilar vessels and bile ducts; rubber hose for attaching specimens to perfusion apparatus, cotton wads for plugging hepatic veins, and ligature with needle to secure nozzles. Two identification tags are also shown. (B) Cirrhotic liver with nozzle tied into portal vein. Adapted with permission from ref. (10).

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