Orths Solution

1. Indications. General purpose fixative but most suitable for the demonstration of chromaffin granules in adrenal medulla and pheochromocytomas.

2. Composition: 2.5 g potassium dichromate (K2Cr2O7), 1.0 g sodium sulfate (Na2SO4), 100 mL distilled water.

Just before use, add 10 mL concentrated formalin.

3. Procedure. Fix slices not thicker than 4 mm in 20 volumes of fixative. The fixation time will be about 24 h to 48 h. Wash in running water for 24 h. Transfer to 70% ethyl alcohol.

4. Storage. Store in 70% alcohol.

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