Kaiserlings Solutions

1. Composition.

a. Kaiserling I: 85 g potassium acetate, 45 g potassium nitrate (KNO3), 4,800 mL formalin solution (3-4%).

b.Kaiserling II: ethyl alcohol, 80-95%.

c. Kaiserling III: 200 g potassium acetate, 300 mL glycerin, 900 mL tap water.

2. Procedure. Fix specimen for 1-5 d in Kaiserling I. Fixation time will vary with the thickness of the organ. Excessive perfusion with Kaiserling I solution causes loss of natural color because too much blood is rinsed out. Transfer to Kaiserling II for a few hours. Acid hematin will turn into alkaline hematin, which approximates the color of hemoglobin.

3. Mounting. Use Kaiserling III solution.

MODIFIED KAISERLING'S SOLUTION AFTER LUNDQUIST (6) This method was developed to avoid the use of alcohol which tends to add to the stiffening and contraction of the specimens.

1. Composition.

a. Kaiserling I: 200 g potassium acetate, 45 g potassium nitrate (KNO3), 80 g chloral hydrate, 444 mL formalin 4,000 mL tap water.

2. Procedure. Suspend the specimen in 10-20 times its volume of fluid. Just after the fixation is completed (avoid overfixation), wash thoroughly in running water and retrim so that all cut surfaces are resurfaced. Transfer to mounting solution for 12 h. Change solution for permanent mounting.

3. Mounting: Kaiserling III: 10 g potassium acetate, 5 g chloral hydrate, 10 mL glycerin, 90 mL tap water.

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Responses

  • liam
    What is kaiserlings technique?
    3 years ago
  • kenneth
    What is a kaiserling technique?
    3 years ago
  • delmina
    What is kaiserling preparation?
    3 years ago
  • ULLA-MAJ KARLSSON
    What is kaiserling solution?
    3 years ago
  • phillipp
    How us kaiserling pulvertaft solution prepared?
    2 years ago
  • timotea
    What is kaiserling fluid?
    2 years ago
  • Welde
    What are the content of kaiserling solution 1?
    2 years ago
  • scott
    Why is potassium acetate used in kaiserling?
    2 years ago
  • Saverio
    Can kaiserling?
    2 years ago
  • yorda
    What is kaserling solution used for?
    1 year ago
  • john
    What is kaiserlings solution?
    5 months ago
  • prudenzio
    What is tge function of potassium nitrate in kaiserling's fluid?
    3 months ago
  • Susan Wall
    How do you fix a specimen using kaiserling method?
    2 months ago

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