Hellys Fixative

1. Indications. This is an excellent fixative for bone marrow and organs containing much blood. It is superior to Zenker's fixative in terms of penetration. Still, most hema-topathologists now prefer the B5-fixative.

2. Composition. 2.5 g potassium dichromate (K2Cr2O7), 5.0 g mercuric chloride (HgCl2), 1.0 g sodium sulfate, anhydrous (Na2SO4), 100 mL distilled water, 5-6 mL formalin.

3. Procedure. Fix slices not thicker than 6 mm in 20 volumes of fixative. The fixation time will be about 12-24 h. Tissues must then be washed for 14-16 h. Transfer to 80% alcohol. Residues of mercuric chloride must be removed from the sections with 0.5% aqueous iodine (5 min) followed by 5% aqueous sodium thiosulfate (5 min).

4. Storage. Use 70% ethyl alcohol for short-term storage. For long-term preservation, dehydration and paraffin embedding are the methods of choice.

Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

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