Glutaraldehyde

1. Indications. This is a fixative for electron microscopic studies and certain histochemical methods (see below)

but phosphate-buffered glutaraldehyde also can be used as an all-purpose fixative. Glutaraldehyde produces less irritating fumes than formalin, is well-suited for perfusion of large specimens, and yields excellent cytological details. Connective tissue stains are well differentiated. The dye uptake is increased in glutaraldehyde-fixed sections. Sectioning artifacts are less frequent. The fixative is more expensive than formalin.

2. Composition. The final preparation represents a 2% glutaraldehyde solution: 50 mL purified glutaraldehyde, 25%, 575 mL Sorenson's phosphate buffer 0.1 M, pH 7.4.

3. Procedure. Fix slices not thicker than 4 mm in 20 volumes of 4% glutaraldehyde solution. The fixation time at room temperature will be 6-24 h. Cold fixation with glutaraldehyde for histochemical enzyme location yields complete fixation after 6 h but only in the outer 1 mm of tissue.

4. Storage. Use 2% or 4% glutaraldehyde solution; store at 4°C.

Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

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