From Histological Sections

1. Deparaffinize sections through two changes each of xylene, absolute alcohol, and 95% alcohol.

2. Rinse well in distilled water.

3. Place slides for 5-10 min in freshly made up bleaching solution, consisting of 25 mL hydrogen peroxide 3%, 25 mL acetone, and 1 drop ammonium hydroxide.

4. Wash well in running tap water and distilled water.

5. Stain as desired.


1. Carson FL. Histotechnology. A Self-Instructional Text. ASCP Press, American Society of Clinical Pathology, Chicago, IL, 1990.

2. Luna LG. Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts. Johnson Printers, Downer's Grove, IL, 1992.

3. Sheehan DC, Hrapchak BB. Theory and Practice of Histotechnology, 2nd ed. CV Mosby Company, St. Louis, MO, 1980.

4. Clark WE. Osteomalacia, histopathologic diagnosis made simple (letter to the editor). Am J Clin Pathol 1976;66:1025-1026.

5. Lillie RD. Histopathologic Technic and Practical Histochemistry, 3rd ed. McGraw-Hill, New York, 1965.

6. Ehrenreich T, Espinosa T. Chromotrope silver methenamine stain of glomerular lesions. Am J Clin Pathol 1971;56:448-451.

7. Bancroft JD, Stevens A. Theory and practice of histological techniques. Churchill Livingstone, New York, 1982.

8. Thompson SW, Hunt RD. Selected Histochemical and Histopatho-logical Methods. Charles C. Thomas, Springfield, IL, 1966.

9. Putt FA. Manual of Histopathological Staining Methods. John Wiley & Sons, New York, 1972.

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