Bouins Fixative

1. Indications. May serve as a general-purpose fixative but proper use is time-consuming. Excellent for subsequent trichrome staining. Glycogen is retained. Erythrocytes are lysed. Excellent for histologic demonstration of pulmonary edema fluid. Recommended for immunohisto-chemical studies (see below).

2. Composition. Stock solution: 750 mL saturated aqueous picric acid, 250 mL formaldehyde solution (36-40%).

Preparation of aqueous picric acid for stock solution: 20 g picric acid (trinitrophenol, USP), 1,000 mL distilled water.

Heat until picric acid dissolves. Cool and decant super-nate. Prepare fixative just before use, by mixing: 95 mL stock solution, 5 mL glacial (99.7%) acetic acid.

3. Procedure. Fix slices not thicker than 3-5 mm. If the tissue is very soft, thin slices can be cut from larger pieces after about 2 hours of fixation. The fixation must be completed in 12-24 h. Transfer to 50% ethyl alcohol for another 6-24 h. The alcohol should be changed when it becomes yellow.

4. Storage. Fixed tissue should be stored in 70% alcohol.

Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

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