Alcohol

1. Indications. Preservation of urates, glycogen, sulfhydryl groups of protein, and water-soluble pigments; enzyme studies. If alcohol is used to preserve water-soluble substances, no aqueous staining procedures can be used.

2. Composition. Absolute ethyl alcohol.

3. Procedure. Fix slices, not thicker than 5-6 mm, in 20 volumes of absolute alcohol. The fixation time will be about 4 h. Transfer to 70% ethyl alcohol for another 72 h. For enzyme studies, 70% alcohol should not be used, but instead use two additional treatments (12 h each) with absolute ethyl alcohol.

4. Storage. Fixed tissue should be stored in 70% alcohol.

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