Mutant Hunts: To Select or to Screen (Perhaps Even by Brute Force)
MacKay, V. & T.R. Manney (1974a) Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. I. Isolation and phenotypic characterization of nonmating mutants. Genetics 76: 255-271.
MacKay, V. & T.R. Manney (1974b) Mutations affecting sexual conjugation and related processes in Saccharomyces cerevisiae. II. Genetic analysis of nonmating mutants. Genetics 76: 273-288.
Complementation Analysis: How Many Genes are Involved?
Hartwell, L.H. (1980) Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptice mating hormone. J. Cell Biol. 85: 811-822.
Gene Isolation and Characterization
Burkholder, A.C. & L.H. Hartwell (1985) The yeast «-factor receptor: structural properties deduced from the sequence of the STE2 gene. Nucleic Acids Res. 13: 8463-8475.
Whiteway, M., L. Hougan, D. Dignard, D.Y. Thomas, L. Bell, G.C. Saari, F.J. Grant, P. O'Hara, & V.L. MacKay (1989) The STE4 and STE18 genes of yeast encode potential and subunits of the mating factor receptor-coupled G protein. Cell 56: 467-477.
Blinder, D., S. Bouvier, & D.D. Jenness (1989) Constitutive mutants in the yeast pheromone response: ordered function of the gene products. Cell 56: 479-486.
Cairns, B.R., S.W. Ramer, & R.D. Kornberg (1992) Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the 11 kinase and the multiple phosphorylation of the 7 kinase. Genes Dev. 6: 1306-1318.
Gene Identification, Isolation, and Characterization (A New Selection Method)
Chang, F. & I. Herskowitz (1990) Identification of a gene necessary for cell cycle arrest by a negative growth factor of yeast: FAR1 is an inhibitor of a G1 cyclin, CLN2. Cell 63: 9991011.
Ramer, S.W. & R.W. Davis (1993) A dominant truncation allele identifies a gene, STE20, that encodes a putative protein kinase necessary for mating in Saccharomyces cerevisiae. Proc. Natl Acad Sci. USA 90: 452-456.
Leberer, E., D. Dignard, D. Harcus, L. Hougan, M. Whiteway, & D.Y. Thomas (1993) Cloning of Saccharomyces cerevisiae STE5 as a suppressor of a Ste20 protein kinase mutant: structural and functional similarity of Ste5 and Farl. Mol. Gen. Genet. 241: 241254.
Hasson, M.S., D. Bllinder, J. Thorner, & D.D. Jenness (1994) Mutational activation of the STE5 gene product bypasses the requirement for G protein ¡3 and 7 subunits in the yeast pheromone response pathway. Mol. Cell. Biol. 14: 1054-1065.
Akada, R., L. Kallal, D.I. Johnson, & J. Kurjan (1996) Genetic relationships between the G protein ih, complex, Ste5p, Ste20p and Cdc42p: investigation of effector roles in the yeast pheromone response pathway. Genetics 143: 103-117.
Choi, K-Y., B. Satterberg, D.M. Lyons, & E.A. Ellion (1994) Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisieae. Cell 78: 499-512.
Whiteway, M„ K.L. Clark, E. Leberer, D. Dignard, & D.Y. Thomas (1994) Genetic identification of residues involved in association of a and ¡3 G-protein subunits. Mol. Cell. Biol. 14: 3223-3229.
Advanced Concepts in Molecular Genetic Analysis—Mutation Analysis
Valtz, N., M. Peter, & I. Herskowitz (1995) FAR! is required for oriented polarization of yeast cells in response to mating pherones. J. Cell Biol. 131: 863-873.
Butty, A.-C., P.M. Pryciak, L.S. Huang, I. Herskowitz, & M. Peter (1998) The role of Farlp in linking the heterotrimeric G protein to polarity establishment proteins during yeast mating. Science 282: 1511-1516.
Erdman, S., L. Lin, M. MaJczynski, & M. Snyder (1998) Pheromone-regulated genes required for yeast mating differentiation. J. Cell Biol. 140: 461-483.
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