Mutant Hunts: To Select or to Screen (Perhaps Even by Brute Force)
Novick, P. & R. Schekman (1979) Secretion and cell-surface growth are blocked in a temperature-sensitive mutant of Saccahromyces cerevisiae. Proc. Natl Acad. Sci. USA 76: 1858-1862.
Complementation Analysis: How Many Genes are Involved?
Novick, P., C. Field, & R. Schekman (1980) Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell 21: 205-215.
Mutant Hunts and Complementation Analysis (Continued)
Deshaies, R.J. & R. Schekman (1987) Yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum. J. Cell Biol. 105: 633-645.
Novick, P., S. Ferro, & R. Schekman (1981) Order of events in the yeast secretory pathway. Cell 25: 461-469.
Kaiser, C.A. & R. Schekman (1990) Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell 61: 723-733.
Gene Isolation, Characterization, and Multiple Alleles
Normington, K., K. Kohno, Y. Kozutsumi, M-J. Gething, & J. Sambrook (1989) S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP. Cell 57: 1223-1236.
Sadler, I., A. Chiang, T. Kurhara, J. Rothblatt, J. Way, & P. Silver (1989) A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein. J. Cell Biol. 109: 2665-2675.
Feldman, D., J. Rothblatt, & R. Schekman (1992) Topology and functional domains of Sec63p, an endoplasmic reticulum membrane protein required for secretory protein translocation. Mol. Cell. Biol. 12: 3288-3296.
Kurihara, T. & P. Silver (1993) Suppression of a sec63 mutation identifies a novel component of the yeast endoplasmic reticulum translocation apparatus. Mol. Biol. Cell 4: 919-930.
Elrod-Erickson, M.J. & C.A. Kaiser (1996) Genes that control the fidelity of endoplasmic reticulum to Golgi transport identified as suppressors of vesicle budding mutations. Mol. Biol. Cell 7: 1043-1058.
Rothblatt, J.A., R.J. Deshaies, S.L. Sanders, G. Daum, & R. Schekman (1989) Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast. J. Cell Biol. 109: 2641-2652.
More Suppression and Enhancement
Scidmore, M.A., H.H. Okamura, & M.D. Rose (1993) Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum. Mol. Biol. Cell 4: 1145-1159.
Roberg, K.J., S. Bickel, N. Rowley, & C.A. Kaiser (1997) Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8. Genetics 147: 1569-1584.
Gene Isolation and Characterization
Espenshade, P., R.E. Gimeno, E. Holzmacher, P. Teung, & C.A. Kaiser (1995) Yeast SEC16 gene encodes a multidomain vesicle coat protein that interacts with Sec23p. J. Cell Biol. 131: 311-324.
Gimeno, R.E., P. Espenshade, & C.A. Kaiser (1996) COPII coat subunit interactions: Sec24p and Sec23p bind to adjacent regions of Secl6p. Mol. Biol. Cell 7: 1815-1823.
Shaywitz, D.A., P.J. Espenshade, R.E. Gimeno, & C.A. Kaiser (1997) COPII subunit interactions in the assembly of the vesicle coat. J. Biol. Chem. 272: 25413-25416.
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