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Tu, J., W. Song, & M. Carlson (1996) Protein phosphatase type 1 interacts with proteins required for meiosis and other cellular processes in Saccharomyces cerevisiae. Mol. Cell. Biol. 16: 4199-4206.

1. Diagram the bait fusion gene, the library fusion gene, and the reporter gene constructs.

2. Gipl protein is a novel protein identified in this study.

(a) What part of the 573-residue Giplp is responsible for binding to Glc7p?

(b) List all of the phenotypes of the gipl null mutation.

(c) At what time during sporulation (which takes about 18-24 hours) is GIP1 expressed?

(d) Is this consistent with the phenotype of gipl mutations? In what way?

(e) Why do the authors suggest that Gipl protein is a regulator of late meiotic gene expression?

3. Several GIP genes were identified in this study.

(a) List the genes other than GIP1 and GIP2 that were identified by this two-hybrid analysis as Glc7p interacting proteins.

(b) These genes were not novel but had been identified previously by genetic analysis. What cellular function is proposed for each?

(c) What results in Table 3 indicate that Giplp, Redlp, and Scd5p (poorly) compete with Reglp but not Gaclp for an overlapping binding site on Glc7p?

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