Table

Schematic of the IMAC Purification Protocol

Sample Injection Step Gradient 1 Step Gradient 2 Linear Gradient 3 Fraction Collection

50 mL injection; 0.2 mL/min flow rate 2% IMAC-B for 15 column volumes 5% IMAC-B for 15 column volumes 5% to 30% IMAC-B over 20 column volumes 1 mL fractions

Fig. 2. Typical chromatogram of an IMAC purification. The dashed line represents the gradient used in the purification. The solid line is the absorbance (A280) of the eluted protein with the fractions containing the Sec23/24 complex shown on the inset silver stained gel. The relative percent of IMAC Buffer B is shown with the dashed line.

Fig. 2. Typical chromatogram of an IMAC purification. The dashed line represents the gradient used in the purification. The solid line is the absorbance (A280) of the eluted protein with the fractions containing the Sec23/24 complex shown on the inset silver stained gel. The relative percent of IMAC Buffer B is shown with the dashed line.

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