Table of Contents

Arrays of Transfected Mammalian Cells for Holger Erfle and High Content Screening Microscopy Rainer Pepperkok 1 2. Imaging Platforms for Measurement of Rainer Pepperkok, Membrane Trafficking Jeremy C. Simpson, Jens Rietdorf, Cihan Cetin, Urban Liebel, Stefan Terjung, and Timo Zimmermann 8 3. Bioinformatic ''Harvester'' A Search Engine Urban Liebel, for Genome-Wide Human, Mouse, and Rat Bjoern Kindler, and Protein Resources Rainer Pepperkok 19 4....

[2 Imaging Platforms for Measurement of Membrane Trafficking

Simpson, Jens Rietdorf, Cihan Cetin, Urban Liebel, Stefan Terjung, and Timo Zimmermann In this chapter we describe automated imaging methods used to measure the transport of an established membrane transport marker from the endoplasmic reticulum to the plasma membrane. The method is fast and significantly robust to be applied in systematic studies on a large scale such as genome-wide screening projects. We further describe the use of software macros and plugins in...

[3 Bioinformatic Harvester A Search Engine for Genome Wide Human Mouse and Rat Protein Resources

By Urban Liebel, Bjoern Kindler, and Rainer Pepperkok Abstract Harvester is a meta search engine for gene and protein information. It searches 16 major databases and prediction servers and combines the results on pregenerated HTML pages. In this way Harvester can provide comprehensive gene-protein information from different servers in a convenient and fast manner. The Harvester search engine works similar to Google, offering genome-wide ranked results at very high speed. Here we describe how to...

Contributors to Volume 404

Article numbers are in parentheses following the name of contributors. Affiliations listed are current. Yoshikatsu Aikawa 37 , Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences at Kagawa, Tokushima-bunri University, Saniki-ku, Kagawa, Japan Bruno Antonny 10 , Institut de Pharmacologie, Moleculaire et Cellulaire, CNRS, Valbonne, France Meir Aridor 11 , Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania Brian...

[4 Microinjection as a Tool to Explore Small GTPase Function

Microinjection Brain Tissu

Microinjection overcomes the plasma membrane barrier to the introduction of charged or large nonlipid soluble molecules into cells by the direct insertion of a hollow capillary micropipette into the cell. With the application of pressure, aqueous solution is then directly transferred into either the cytosol or the nucleus. I give specific examples of the application of this approach to the functional study of small GTPases of the Sar1, ARF, and rab family in membrane trafficking between the...