Chapter 1 Strategies for Novel Research Projects

Chapter 2 Rapid Isolation of Specific cDNAs or Genes by PCR

Chapter 3 Construction and Screening of Subtracted and Complete Expression cDNA Libraries

Chapter 4 Subcloning of Genes or DNA Fragments

Chapter 5 Nonisotopic and Isotopic DNA or RNA Sequencing

Chapter 6 Information Superhighway and Computer Databases of Nucleic Acids and Proteins

Chapter 7 Characterization of DNA or Genes by Southern Blot Hybridization

Chapter 8 Gene Overexpression by Sense RNA in Mammalian Systems

Chapter 9 Gene Underexpression in Cultured Cells and Animals by Antisense DNA and RNA Strategies

Chapter 10 Analysis of Gene Expression at the Functional Genomic


Chapter 11 Analysis of Gene Expression at the Proteomic Level

Chapter 12 Analysis of Cellular DNA or Abundance of mRNA by Radioactivity in Situ Hybridization (RISH)

Chapter 13 Localization of DNA or Abundance of mRNA by Fluorescence in Situ Hybridization (FISH)

Chapter 14 In Situ PCR Hybridization of Low Copy Genes and in Situ RT-PCR Detection of Low Abundance mRNAs

Chapter 15 Isolation and Characterization of Genes from Genomic DNA Libraries

Chapter 16 Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression

Chapter 17 New Strategies for Gene Knockout

Chapter 18 Large-Scale Expression and Purification of Recombinant Proteins in Cultured Cells

Chapter 19 Quantitative Analysis of Functional Genome by Current Real-Time RT-PCR

Chapter 20 High-Throughput Analysis of Gene Expression by Cutting-Edge Technology — DNA Microarrays (Gene Chips)

Chapter 21 Construction and Screening of Human Antibody Libraries: Using State-of-the-Art Technology — Phage Display

Chapter 22 Down-Regulation of Gene Expression in Mammalian Systems via Current siRNA Technology

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