This chapter presents a strategy for the design of an HPLC assay system. Section 4.1, Setting up the Assay, focuses on the enzymatic reaction and the steps leading to the development of the assay. These steps are previewed in Table 4.1. Section 4.2, The Use of HPLC to Establish Optimal Conditions for the Enzymatic Reaction, discusses the procedure for monitoring the activity of an enzyme with the HPLC method and the use of HPLC assays to determine the parameters required for obtaining optimal activity.

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