Ketoglutarate reductase catalyzes the formation of saccharopine from lysine, which is the first step in a major pathway for lysine catabolism. NADPH and a-ketoglutarate are cofactors in the reaction.
The o-phthaldialdehyde derivatives of lysine and saccharopine were separated on a Ultrasphere-XL ODS column (4.6 mm x 70 mm, 3 fim). The column was equilibrated using methanol-0.1 M sodium acetate (pH 6.7) in a 16:84 ratio. The flow rate was 1.6 mL/min. After injection, methanol was increased linearly to 20% in 0.5 minute. A further increase to 20% methanol occurred at 11.5-12 minutes. A return to 16% methanol occurred between 19 and 19.5 minutes, and the column was equilibrated for 8.5 minutes prior to
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