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Fig. 3. ClustalW alignments of VDE proteins (left) and HO proteins (right) compared among S. cerevisiae, S. castellii, C. glabrata and Z. rouxii. The endonuclease domain of each protein lies between the N- and C-terminal parts of the protein-splicing domain, which are shaded. Circles mark conserved cysteine residues in the zinc finger of HO

in the late mitotic G1 phase of haploids. The complex way in which HO activity is regulated is reasonably well understood (Cosma 2004), whereas how VDE activity is restricted to meiosis - even though the protein is expressed at high levels in mitotically grown haploid or diploid cells - is unknown (Gim-ble and Thorner 1992).

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