Figure 265

Mitotic spindle in metaphase. Using indirect immunofluorescence techniques, the mitotic spindle in a Xenopus XL-177 cell was labeled with an antibody against a-tubulin conjugated with fluorescein (green). DNA was stained blue with fluorescent DAPI stain. In metaphase, the nuclear membrane disassembles, DNA is condensed into chromosomes, and microtubules form a mitotic spindle. The action of microtubule-associated motor proteins on the microtubules of the mitotic spindle creates the metaphase plate along which the chromosomes align in the center of the cell, x 1,400. (Courtesy of Dr. Thomas U. Mayer.)

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