Figure 232

Electron micrograph of Golgi cisternae. a. This transmission electron micrograph shows a quick-frozen isolated Golgi apparatus replica from a cultured CHO cell line. The frans-Golgi cisternae are in the process of coated vesicle formation, b. Incubation of the frans-Golgi cisternae with the coatomer-depleted cytosol shows a decrease in vesicle formation activity. Note the lack of vesicles and the fenestrated shape of the frans-Golgi cisternae. x85,000. (Courtesy of Dr. John E. Heuser, Washington University School of Medicine.)

constitutive transport vesicle lysosome

secretory storage vesicle

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