Figure 206

This specimen of the pars distalis is stained with brilliant crystal scarlet, aniline blue, and Martius yellow to distinguish the various cell types and connective tissue stroma. The cords of cells are surrounded by a delicate connective tissue stroma stained blue. The sinusoidal capillaries are seen in close association with the parenchyma and contain erythrocytes stained yellow. In the region shown here, the acidophils (Ac) are the most numerous cell type present. Their cytoplasm stains cherry red. The basophils (Bas) stain blue. The chromophobes (Ch), though few in number in this particular region, are virtually unstained. x640.

Percentage

Cell Type of Total Cells General Staining Specific Staining Product

Somatotrope (GH cell) 50

Lactotrope (PRL cell) 15-20

Corticotrope (ACTH cell) 15-20

Gonadotrope (FSH and 10

LH cells)

Thyrotrope (TSH cell) -5

Acidophil Acidophil

Basophil

Basophil

Basophil

Orange G (PAS -) Herlant's erythrosin Brooke's carmosine

Lead hematoxylin (PAS +)

Aldehyde-fuchsin Aldehyde-thionine (PAS +)

Aldehyde-fuchsin Aldehyde-thionine (PAS +)

Growth hormone (GH) Prolactin (PRL)

Proopiomelanocortin (POMC), which is cleaved in human into adrenocorticotropic hormone (ACTH) and /Mipotrophic hormone (jS-LPH)

Follicle-stimulating hormone (FSH) and luteinizing hormone (LH)

Thyrotropic hormone (TSH)

fact has led to definitive identification of specific cell types by immunocytochemistry. These studies have classified cells of anterior lobe of the pituitary gland into five cell types:

table 20.2. Staining Characteristics of Cells Found in the Anterior Lobe of the Pituitary Gland

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