Enamel organ cells and odontoblasts in a developing tooth. This photomicrograph of an unstained plastic thick section viewed with the phase contrast microscope shows enamel organ cells and odontoblasts as they begin to produce enamel (E) and dentin (D), respectively. Young enamel is deposited by secretory-stage ameloblasts (SA) onto the previously formed dentin. The enamel appears dark in the illustration. At the top, the enamel surface displays a characteristic picket-fence pattern because of the sharp contrast between the lightly stained Tomes' processes (TP) of the secretory-stage ameloblasts and the darkly stained young enamel product that partly surrounds the cell processes. The nuclei (N) at the right belong to cells of the stratum intermedium. The nuclei (N) on the left belong to odontoblasts located in the basal part of the cells. The odontoblast cytoplasm extends to the dashed line. At this point, cytoplasmic processes (OP) extend into the dentin. X85.
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