Trephine Biopsy

There are several reusable and disposable commercially available instruments for performing a bone marrow trephine biopsy procedure in adult and paediatric patients. The choice is based on safety, convenience and quality of the specimen obtained.

Smaller-gauge needles are used in children and patients with severe osteoporosis. The procedure should be done under sterile conditions. The patients are given suitable local anaesthesia/analgesia and positioned properly for the procedure. The posterior superior iliac spines are the most favoured sites for aspiration. Anterior iliac crest or sternal puncture are also used. The aim is to obtain a biopsy specimen of adequate length (usually 1-1.5 cm in length) avoiding crushing and excess haemorrhage.

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