In cases of neoplastic disease, the histology should represent the tumour, its relationship to the adjacent mucosa and underlying bone or cartilage. Focal abnormalities of mucosa need to be sampled.
• three blocks of tumour to illustrate the interface with adjacent normal tissues.
• three blocks of adjacent mucosa.
• closest deep surgical margin.
• samples of other lesions, e.g., nodules or polyps.
• in intact specimens, sample the mucosal margins before sawing the bone and submit separately (reduces contamination of the margins). Cut with a sharp blade firmly down to bone and use a flat blunt instrument to dissect mucosa free from the bone.
• in intact specimens, saw the bone into 0.5 cm slices in the transverse plane (vertical plane in vivo).
• if fragmented, bread-slice larger specimens and submit as labelled blocks. Microscopic analysis may allow reconstruction and useful assessment of margins.
Was this article helpful?