Validity

Any useful assay of ROS production should not interfere with mitochondrial function. This can be checked by measuring mitochondrial respiration in the presence of the molecular probes constituting the chemical detection system. Addition of horseradish peroxidase and homo-vanillic acid, at the same final concentration used in the fluorometric H2O2 assay, does not change the rates of mitochondrial state 4 or state 3 respiration or the RCI. High purity horseradish peroxidase and superoxide dis-mutase should be used. Commercial solution of these two enzymes stabilized with agents capable of altering mitochondria should be avoided. The appropriateness of the particular commercial reactants used can be easily checked in pilot experiments measuring mitochondrial respiration in their absence and in their presence.

Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

Get My Free Ebook


Post a comment