Trap Assay

The telomerase repeat amplification protocol (TRAP) assay, first described by Kim et al. (1994), relies upon primer extension of an oligonucletide by telomerase. Cells are lysed and cellular protein extracts are incubated with an oligonucletide to which a series of TTAGGG repeats will be added when telomerase is present in the cell extract. Variations of the TRAP assay exist, including radioactive or nonradioactive gel-based detection, ELISA-based detection and semiquantitative or quantitative protocols. For an excellent review of the TRAP assay and many of its iterations, see Saldanha et al. (2003).

Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

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