Table 434

Effects of aging and hormone treatment on progesterone receptor (PR) expression in the hypothalamus of aging rats

Rat strain

OVX duration before steroid treatment

Steroid hormone treatment

Technique

Effect on progesterone receptor (PR)

Sprague-Dawley

7 d

17P-E2 capsule 2 d ) P 0.2 mg/kg s.c. 17P-E2 4 d )P 0.2 mg/kg s.c.

Nuclear exchange assay to determine DNA content of POA, MBH, and AMYG of Y and MA.

E2 (2d): * PR binding sites in POA and MBH in Y vs. MA. E2 (4d): No age-related change.

Sprague-Dawley

17 d

17P-E2, 3 d

Binding assay in POA, PVP, BNST, VMN, ARC, AMYG of Y, MA, or A.

No age-related differences in induction of PR binding by E2 in any of the observed structures.

Fischer 344

10 d

17P-E2, 4 d

in situ hybridization in POA and MBH of Y, MA, A.

No differences among any age groups.

Long-Evans

Intact

None

in situ hybridization in AVPV, POA, ARC, VMN of Y and MA (early and late PE).

POA: no age difference. VMN/ARC: PR mRNA lower in long-term PE versus early PE and young, proestrus. AVPV: lower PR mRNA in early and long-term PE compared to young.

Modified from Chakraborty and Gore 2004.

Abbreviations: P, Progesterone; POA, Preoptic area; MBH, medial basal hypothalamus; AMYG, amygdala; PVP, periventricular preoptic area; BNST, bed nucleus of the stria terminalis; ARC, arcuate nucleus; VMN, ventromedial nucleus; AVPV, anteroventral periventricular nucleus; d, days; Y, young; MA, middle-aged; A, aged; PE, persistent estrus; 17p-E2 17-beta estradiol.

Modified from Chakraborty and Gore 2004.

Abbreviations: P, Progesterone; POA, Preoptic area; MBH, medial basal hypothalamus; AMYG, amygdala; PVP, periventricular preoptic area; BNST, bed nucleus of the stria terminalis; ARC, arcuate nucleus; VMN, ventromedial nucleus; AVPV, anteroventral periventricular nucleus; d, days; Y, young; MA, middle-aged; A, aged; PE, persistent estrus; 17p-E2 17-beta estradiol.

and treatments that can be used with these animals warrant careful consideration when constructing a study focusing on effects of age-related changes on reproductive function.

Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

Get My Free Ebook


Post a comment