Proteomic Analysis of Posttranslational Modifications

Post-translational protein modifications significantly expand the portfolio of proteins with specific properties encoded by the genetic code. They allow for the production of multiple variants of a single gene product through specific, potentially reversible, covalent alterations of amino acid side chains. To date, about 200 distinct post-translational protein modifications are known (Khidekel and Hsieh-Wilson, 2004). However, we are just at the beginning of a thorough qualitative and quantitative characterization of only a few of these post-translational modifications in the ''aging proteome.'' Importantly, proteins will not necessarily only incorporate a single modification, but often multiple modifications accumulate specifically on long-lived proteins in tissues with low protein turnover (e.g., the crystallins in the lens). Considering the progressive age-dependent functional changes of most cell types and tissues, an accurate profile of age-dependent post-translational protein modifications is mandatory. The following paragraphs will focus on the proteomic characterization of selected post-translational protein modifications such as phosphorylation and oxidation.

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Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

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