Light Microscopy

Samples of the human retina were immediately prefixed in 2% osmium tetroxide at pH 7.4 in veronal-acetate buffer for five minutes at 4°C. After fixation, the specimens were washed with veronal-acetate buffer (pH 7.4), dehydrated in a graded ethanol series and embedded in paraffin. Thin sections (about 4 m) were made for morphological staining with toluidine blue (0.05% for 1 minute). Lipids were stained by means of special histo-chemical techniques for light microscopic analysis. In order to determine the composition and distribution of the lipids, three different stains were used: the bromine-Sudan black B stain, which stains all classes of lipids; bromine-acetone-Sudan black B, which stains only phospho-lipids; and Oil red O, which stains neutral lipids (especially esters of saturated and unsaturated fatty acids).

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Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

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