Insertional Mutagenesis

The insertional inactivation of genes by retroviral mutagenesis has been successfully employed in zebrafish as described above. How will this be useful in longevity selection? The underlying rationale for insertional muta-genesis in aging studies is that the inactivation of genes that have negative influence on the lifespan might increase the lifespan. Since it is easy to detect dominant genes, first dominant screens should be conducted by simply injecting the retroviruses into the Nothobranchius embryos and then the fish should be selected that are long lived and should be used further to identify the genes that are affected. This concept could also be extended further for recessive screens either by diploidization or by three-generation screens to identify the long-lived Nothobranchius. The methods that are used in zebrafish for insertional mutagenesis should be applicable for the annual fish.

How to Stay Young

How to Stay Young

For centuries, ever since the legendary Ponce de Leon went searching for the elusive Fountain of Youth, people have been looking for ways to slow down the aging process. Medical science has made great strides in keeping people alive longer by preventing and curing disease, and helping people to live healthier lives. Average life expectancy keeps increasing, and most of us can look forward to the chance to live much longer lives than our ancestors.

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