Figure 45.4 Enrichment of Leydig cell progenitors by flow cytometry analysis of the Hoechst dim side population. Goodell and colleagues reported the use of Hoechst dye exclusion to select for a population of stem cells based upon their ability to exclude the dye via the multidrug resistance transporter protein. The cells are sorted into SP (side-population), which extrudes the dye, and non-SP which retains the dye. The SP fraction represents about 0.9% of the total population. Briefly, after the Hoechst dye staining, the cell solution is excited with the UV laser at 350 nm. Duo-wavelength filters in the flow cytometer are used to detect the resulting fluorescence (Dakocytomation, Carpinteria, California).

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Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

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