Bloodbrain Barrier Function

Albumin leakage, a quantitative index of endothelial barrier dysfunction, can be applied to the brain for determination of BBB function. Albumin extravasation in the brain can be quantified either by fluorescence imaging of the leakage of fluorescein isothiocyanate-labeled albumin or sulforhodamine (Texas Red)-labeled albumin from cerebral venules or from the clearance of the same fluorochromes from blood to the artificial CSF perfusing the brain surface. With the imaging approach, fluores-cently tagged albumin is administered intravenously to the animals 15 minutes before the baseline observation period, and fluorescence intensity (excitation wavelength, 420-490 nm; emission wavelength, 520 nm) is detected using a silicon-intensified target camera. The fluorescence intensities within a specified segment of a cerebral venule (Iv) within the cranial window and in a contiguous area of perivenular interstitium (Ii) are measured at various times after administration of fluorescent-albumin using a computer-assisted digital imaging processor (NIH Image 1.61 on a Macintosh computer). Vascular albumin leakage is determined from the difference in fluorescence intensity between the outside and inside of the venular segment. An index of albumin leakage is determined from the Ii-to-Iv ratio (after subtraction of baseline intensity measured before labeled albumin injection) at specific time points during the course of the experiment.

Was this article helpful?

0 0
Blood Pressure Health

Blood Pressure Health

Your heart pumps blood throughout your body using a network of tubing called arteries and capillaries which return the blood back to your heart via your veins. Blood pressure is the force of the blood pushing against the walls of your arteries as your heart beats.Learn more...

Get My Free Ebook


Post a comment