Pathology Techniques

Pathological characterization of genetically engineered mice includes a complete necropsy for gross tissue evaluation and microscopic evaluation via histopathology. Specialized pathology methods such as immunohistochemistry and flow cytometry should also be utilized to investigate specific lesions, and the necropsy should be supported by clinical pathology findings. Genetically altered mice can and do have unexpected phenotypes. In addition, the phenotype of a particular gene alteration can be expressed in other tissues that may directly or indirectly influence the target tissue of interest. This is true of all genomic manipulations and underscores the importance of accurate and thorough phenotypic characterization and model validation by pathologists familiar with background mouse pathology and the human disease of interest. It is also important to understand that different strains of mice vary in normal anatomy, physiology, and behavior.

0 0

Post a comment