Lean Body Mass

Muscle wasting is a consequence of many systemic diseases such as diabetes, cancer, sepsis, hyperthy-roidism, uremia, muscle disuse, and nerve injury. Thus, muscle mass determination is important to many systemic disease studies. Muscle-specific phenotyping procedures are important and consist of an intensive anatomic evaluation that involves muscle mass determination via gross pathology and histology, followed by biochemical techniques such as molecular flux spectroscopy. Such techniques are beyond the scope of this chapter. At the time of necropsy, the total mouse weight is obtained, and an analysis of muscle weights can be obtained by dissecting out individual muscles. An average of left and right sides should be used. Muscles are examined grossly, and samples are obtained for H&E staining. To better visualize muscle fibers, samples can be double stained with anticaveolin-3 IgG to help demarcate the sarcolemma or plasma membrane and propidium iodide to help demarcate nuclei. Electron microscopy is valuable in examining skeletal muscle disease at the cellular level. A muscle biopsy can also be utilized to investigate clinical manifestations of muscle disease such as muscle wasting. Serum creatinine kinase, a cytosolic muscle enzyme that elevates with lysis or necrosis of muscle fibers, is a good indicator of the extent of muscle wasting.36

Diabetes 2

Diabetes 2

Diabetes is a disease that affects the way your body uses food. Normally, your body converts sugars, starches and other foods into a form of sugar called glucose. Your body uses glucose for fuel. The cells receive the glucose through the bloodstream. They then use insulin a hormone made by the pancreas to absorb the glucose, convert it into energy, and either use it or store it for later use. Learn more...

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