Existing Colonies

Breeding colonies of inbred strains will often be divided into a stem line and an expansion colony (see above). Ideally, the stem line colony will be physically separated from other colonies. If not, it should at least be kept with strains of a different coat color and microsatellite profile. With good physical separation and a small colony size, the chance of contamination is low, so the colony would not need to be monitored frequently. If it is maintained in an isolator with no other strain, then once it has been authenticated, it hardly needs any routine monitoring.

Expansion colonies may be large and at risk from other colonies in the same building. The colony might be monitored two to four times per year, with sample sizes of about ten animals, using a set of markers that will preclude contamination by all other strains in the building.

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