Cryopreservation

Cryopreservation protects against catastrophic losses by such events as breeding cessation, genetic contamination, or disease outbreaks, stops genetic drift, and eliminates the necessity and expense of maintaining live breeders when specific lines are not in high demand. Cryopreserved stocks are pathogen free and can be recovered pathogen free. Cryopreservation of mouse lines can be approached using embryos, semen, or whole ovaries. Embryos take the most effort and expertise to freeze, but take little expertise to thaw and so, are especially suitable for transfer to outside facilities through shipment on dry ice. Semen preservation has the advantage that storage of just a few straws has the capability of generating the line many times over; however, the techniques have been most successful using hybrid animals.

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