Authentication of Newly Established Strains

Ideally, newly established colonies should be tested as soon as possible to ensure that they are of the correct genotype. With mice, control DNA from most strains is available from the Jackson Laboratory (www.jax.org). For rats, samples of DNA may be obtained from colleagues or known holders of the strains. If the colony is being established from a small breeding nucleus, it may be possible to test all animals. In this case, the main aim is to test the authenticity of the strain, though the possibility of contamination by one or more nonstrain animals should not be ruled out. A sample of five to ten animals is probably adequate at this stage, and they should probably be tested at about ten microsatellite loci.

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