Platelet Activation Endpoints

[14C]Serotonin release was the first activation endpoint described using washed platelets (Sheridan et al., 1986). In this method, the washed platelets are incubated with test and control serum or plasma and heparin-buffer in flat-bottomed polystyrene microtiter wells (in duplicate or triplicate), performed on a platelet shaker (shaken, not stirred). After 1 h, the reaction is halted with 100 mL of 0.5% EDTA in phosphated-buffered saline (PBS). The microliter plates are centrifuged at 1000 g

TABLE 3 Pipette Scheme for the HIPA Test
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