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PF4 alone

heparin dalteparin enoxaparin danaparoid

FIGURE 4 Cross-reactivity of HIT-IgG antibodies with PF4 complexed to heparin-like anticoagulants: The fluid-phase EIA was used to assess the degree to which IgG present in HIT sera or plasma bound to PF4 alone, PF4-heparin, PF4-dalteparin (Fragmin), PF4-enoxaparin (Clexane), or PF4-danaparoid (Orgaran). The positive cutoff (dashed line) is three standard deviations above the mean (log transformed) absorbance of the normal samples (triangles) using PF4-heparin. The binding of normal antibodies is indicated by triangles. Circles indicate HIT samples that have been positive (closed circle), negative (open circle), or not tested (speckled circle) in a functional assay with the corresponding drug. Abbreviations: EIA, enzyme immunoassay; HIT-IgG, heparin-induced thrombocytopenia immunoglobulin G; PF4, platelet factor 4. Source: From Newman etal., 1998.

PF4 alone

heparin dalteparin enoxaparin danaparoid

FIGURE 4 Cross-reactivity of HIT-IgG antibodies with PF4 complexed to heparin-like anticoagulants: The fluid-phase EIA was used to assess the degree to which IgG present in HIT sera or plasma bound to PF4 alone, PF4-heparin, PF4-dalteparin (Fragmin), PF4-enoxaparin (Clexane), or PF4-danaparoid (Orgaran). The positive cutoff (dashed line) is three standard deviations above the mean (log transformed) absorbance of the normal samples (triangles) using PF4-heparin. The binding of normal antibodies is indicated by triangles. Circles indicate HIT samples that have been positive (closed circle), negative (open circle), or not tested (speckled circle) in a functional assay with the corresponding drug. Abbreviations: EIA, enzyme immunoassay; HIT-IgG, heparin-induced thrombocytopenia immunoglobulin G; PF4, platelet factor 4. Source: From Newman etal., 1998.

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