Viiwestern Blot

A. This technique is widely used as a confirmatory test for AIDS. The patient's serum is added to HIV antigens bound to the nitrocellulose matrix. A positive reaction is detected by the addition of a labeled, antihuman immunoglobulin antibody, as in the indirect ELISA test.

B. To identify an antigen in a mixture, the components of the mixture are separated by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and "blotted" onto a nitrocellulose matrix. Labeled, known antibody is added to locate and identify the antigen of interest.

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